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BIOSC 172L: Introduction to Biotechnology Lab

BioSc172L introduces students to the laboratory environment.  It meets pre-requisite requirements for BioSc147, BioSc134, BioSc119, and BioSc157/9.

This is an introductory laboratory course in which scientific and biotechnology methodology is taught and used to explore and experiment with topics found in the Introduction to Biotechnology course (BioSc172). It is a brief introduction to the skills and concepts necessary to work in the biotechnology industry, allied health, or related fields. Topics will include: the biology, business, and legal/ethical issues surrounding biotechnology; cells, genes, DNA, proteins, genetic engineering, drug development, biofuels, agriculture, bioremediation, biotechnology company structure, and the regulations affecting the field, and a survey of general methodologies utilized in biotechnology research and manufacturing.

This course serves as a launch-point for many education and career pathways including allied health (nursing), biotechnology, and general education requirements.

At the completion of the course the student will be able to: Formulate and write out appropriate research questions and hypothesis when presented with observation phenomena; List the major steps in the scientific method of investigation; Prepare appropriate data tables and graphs from data collected; Demonstrate the use of general math skills in the application of the scientific method (i.e. computatios, ratios, calculations, conversions, logarithms; Describe, explain and apply the metric system of measurement; Collect and evaluate experimental data to accept or reject hypotheses; Explain the theory of spectrophotometry and demonstrate the proper care and use of the spectrophotometer; Demonstrate appropriate teamwork skills in the laboratory; Describe the characteristics of the cell differentiating between the prokaryotic and eukaryotic cell; Demonstrate the proper technique in using microscopes; Sketch the atomic structure for elements with atomic numbers from 1 to 20; Define pH, acids, bases, and buffers and describe their role in living systems; Differentiate between ionic, covalent, and hydrogen bonds; Compare and contrast the properties of the four major groups of macromolecules associated with living systems; Demonstrate the proper use of pH meters and computer-based pH probes to collect and analyze data collected under varying conditions; Describe the structure and function of enzymes; Evaluate the effect of different environmental factors on the rate of enzyme catalysis; Prepare agarose gels and successfully separate a mixture into constituent molecules using gel electrophoresis and column chromatography; Demonstrate the proper use of micropipettes to measure small quantities of fluids; Compare and contrast anaerobic fermentation and cellular respiration; Differentiate between the light dependent and independent reactions in photosynthesis; Describe antigen-antibody reactions and give examples of how immunology techniques are used in scientific research, medicine, and the biotechnology industry; Demonstrate appropriate skills in the aseptic culturing of cells; Explain the structure of DNA and its role in cellular respiration and protein synthesis; Describe some of the major studies that elaborated the structure and role of DNA in cells; Calculate Mendelian probabilities when given the appropriate pedigree information; List potential chemical and biological hazards in the laboratory and take proper precautions against them.

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